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This mixture was centrifuged at 15,000 r/min for 10 min at room temperature

Thus, it is important that a chelator satisfy criteria that allow it to: (1) transport across physiological barriers into compartments where a toxic metal ion is concentrated, (2) form a stable complex with the metal after removing it from the biological chelator, if required at the site and (3) form a chelation complex whose properties render it non-toxic and facilitate its excretion, not only from the site of deposition, but also from the body [3]

PMID 17557503

DCs function as regulators, modulating bone immunity and the vascular microenvironment through antigen presentation and cytokine secretion (85)